Biophysics of Cell-Substrate Interactions Under Shear

Biophysics of Cell-Substrate Interactions Under Shear

Blog Article

Cells adhere to substrates through mechanosensitive focal adhesion complexes.Measurements that probe how cells detach from substrates when they experience an applied force connect molecular-scale aspects of cell adhesion with the biophysical properties of adherent cells.Such forces can be applied through shear devices that flow fluid in a controlled manner across cells.

The signaling pathways associated with Ski de fond - Enfant - Skis - Skate focal adhesions, in particular those that involve integrins and receptor tyrosine kinases, are complex, receiving mechano-chemical feedback from the sensing of substrate stiffness as well as of external forces.This article reviews the signaling processes involved in mechanosensing and mechanotransduction during cell-substrate interactions, describing the role such signaling plays in cancer metastasis.We examine some recent progress in quantifying the strength of these interactions, describing a novel fluid shear device that allows for the visualization of the cell and Toasters its sub-cellular structures under a shear flow.

We also summarize related results from a biophysical model for cellular de-adhesion induced by applied forces.Quantifying cell-substrate adhesions under shear should aid in the development of mechano-diagnostic techniques for diseases in which cell-adhesion is mis-regulated, such as cancers.